mycbase

MYCbase: A Database of MYC

Browse Result

Methylation: 29 Records
Record No:1

DB_ACCESS_NO 80000
REGION MDS:exons 2 and 3, ANLL:exon 2 and exon 3
SEQUENCE N/A
STATUS MDS: hypomethylated; ANLL: exon 2 hypermethylated and exon 3 hypomethylated
CELL_LINE monocyte cell layer harvested from bone marrow or peripheral blood samples patients
OUTCOME transformation process from myelodysplasia to acute leukemia
DISEASE MDS and ANLL
PUBMED 17472275
METHOD methylation-specific enzymes

Record No:2

DB_ACCESS_NO	80001
REGION	entire gene
SEQUENCE	12 CCGGs of entire c-myc gene
STATUS	normal:methylated internal cytosine, tumor: some are unmethylated, treatment: some remethylated
CELL_LINE	normal lung tissue and lung tumor of Female strain A/J mice
OUTCOME	hypomethylation in tumor state which is reversed by budesonide
DISEASE	lung tumor
PUBMED	12325039
METHOD	Restriction digestion followed by southern blot

Record No:3

DB_ACCESS_NO	80002
REGION	entire gene
SEQUENCE	CCGG sequence
STATUS	hypomethylation after administering Wy-14,643 a liver carcinogen
CELL_LINE	B6C3F1 mice liver DNA
OUTCOME	hypomethylation due to carcinogens compared with control mice induces proliferation
DISEASE	liver cancer
PUBMED	11857776
METHOD	Restriction digestion followed by southern blot

Record No:4

DB_ACCESS_NO	80003
REGION	entire gene
SEQUENCE	CCGG sequence
STATUS	hypomethylation after administering DCA, TCA, TCE
CELL_LINE	B6C3F1 mice liver DNA
OUTCOME	hypomethylation due to Wy-14,643 compared with control mice giving rise to liver cancer
DISEASE	liver cancer
PUBMED	10774822
METHOD	Restriction digestion followed by southern blot

Record No:5

DB_ACCESS_NO	80004
REGION	exon 3
SEQUENCE	CCGG sequence
STATUS	hypomethylation of the sequence in B cell malignancy patients
CELL_LINE	Peripheral blood samples from patients
OUTCOME	hypomethylation leads to development of malignancy
DISEASE	acute myeloid leukaemia (AML) and chronic myelomonocytic leukaemia (CMML)
PUBMED	10071077
METHOD	Restriction digestion followed by southern blot

Record No:6

DB_ACCESS_NO	80005
REGION	entire gene
SEQUENCE	N/A
STATUS	hypomethylation
CELL_LINE	tissue samples of urinary bladder tumors and blood
OUTCOME	local hypermethylation potential mechanism for increasing genetic alterations in bladder cancer formation
DISEASE	bladder cancer
PUBMED	9076471
METHOD	Restriction digestion followed by southern blot

Record No:7

DB_ACCESS_NO	80006
REGION	entire gene
SEQUENCE	CCGG sequence
STATUS	hypomethylation
CELL_LINE	tissue samples from tumors
OUTCOME	strong correlation between DNA hypomethylation and gastric carcinogenesis
DISEASE	gastric cancer
PUBMED	9275356
METHOD	Restriction digestion followed by southern blot

Record No:8

DB_ACCESS_NO	80007
REGION	exon 1
SEQUENCE	Hpa II site of c-myc exon I
STATUS	methylation in response to MNNG abd UV
CELL_LINE	No information available
OUTCOME	Possible contribution of methylation to less repair after action of carcinogens
DISEASE	cancer
PUBMED	9095415
METHOD	methylation-specific enzymes

Record No:9

DB_ACCESS_NO	80008
REGION	myc promoter region
SEQUENCE	N/A
STATUS	no difference in methylation pattern between control and treated rats
CELL_LINE	Sprague-Dawley rat liver tissue
OUTCOME	betaine had no effect on methylation of c-myc by MSP and there were no differences in c-myc methylation among the five groups of rats
DISEASE	liver cancer
PUBMED	19642983
METHOD	methylation-specific PCR

Record No:10

DB_ACCESS_NO	80009
REGION	3' end of c-myc gene
SEQUENCE	CCGG
STATUS	hypomethylation in HCC as compared to normal tissue
CELL_LINE	liver tissue from patients
OUTCOME	hypomethylation of c-myc can reflect the malignlant extent of HCC
DISEASE	Hepatocellular crcinoma HCC
PUBMED	9275498
METHOD	Restriction digestion followed by southern blot

Record No:11

DB_ACCESS_NO	80010
REGION	exon 2, 5' region and promoter
SEQUENCE	N/A
STATUS	exon 2: hypermethylation during in vitro aging, 5' region and promoter: hypomethylation
CELL_LINE	IMR90 cells
OUTCOME	hypermethylation stabilizes gene expression while hypomethylation dysregulates gene expression
DISEASE	aging
PUBMED	7776985
METHOD	Restriction digestion followed by southern blot

Record No:12

DB_ACCESS_NO	80011
REGION	exon 3
SEQUENCE	N/A
STATUS	hypomethylation
CELL_LINE	No information available
OUTCOME	hypomethylation at this site and enhanced expression of the myc protein
DISEASE	myeloma and leukemia
PUBMED	7680737
METHOD	no information available

Record No:13

DB_ACCESS_NO	80012
REGION	entire gene
SEQUENCE	N/A
STATUS	hypermethylation of the c-myc gene
CELL_LINE	liver tumors samples of BCF1 mice and C3H/He mice
OUTCOME	hypermethylation of the c-myc gene is common to aging and early tumor development in liver
DISEASE	aging and liver tumor
PUBMED	8365669
METHOD	no information available

Record No:14

DB_ACCESS_NO	80013
REGION	entire gene
SEQUENCE	N/A
STATUS	no change in methylation status
CELL_LINE	HL-60 cells
OUTCOME	increase in mRNA levels after treatment with drug accompanied with increase in transmethylation metabolites
DISEASE	no information given
PUBMED	1417952
METHOD	Restriction digestion followed by southern blot

Record No:15

DB_ACCESS_NO	80014
REGION	entire gene and around it
SEQUENCE	CCGG sites in and around c-myc gene
STATUS	5'region: hypomethylated, 3' region: hypermethylated but some also hypomethylated
CELL_LINE	blood cells from patients with acute and chronic leukemias and with myelodysplastic syndromes
OUTCOME	hypomethylation in the 3' region due to delayed methylation during transforming events
DISEASE	leukemia
PUBMED	1468540
METHOD	Restriction digestion followed by southern blot

Record No:16

DB_ACCESS_NO	80015
REGION	c-myc DNA with a deletion which includes 5' flanking, exon 1 and intron I
SEQUENCE	CCGG sites
STATUS	hypomethylation in the sample as compared to normal DNA
CELL_LINE	normal white blood cells of a mother and one daughter in a Northern Italian family
OUTCOME	truncated DNA and aberrant methylation pattern is not related to neoplastic transformation
DISEASE	no information given
PUBMED	1923512
METHOD	no information available

Record No:17

DB_ACCESS_NO	80016
REGION	exon 3
SEQUENCE	CCGG
STATUS	hypomethylation of a specific site in the third exon of c-myc in the earliest stages of polyp formation which increases with tumour progression
CELL_LINE	specimens of human colorectal tissue
OUTCOME	hypomethylation contributes to deregulation of proliferation at an early stage in tumorigenesis
DISEASE	colorectal cancer
PUBMED	1586594
METHOD	Restriction digestion followed by southern blot

Record No:18

DB_ACCESS_NO	80017
REGION	intron 1
SEQUENCE	N/A
STATUS	hypomethylation
CELL_LINE	N-nitrosomorpholine-induced rat liver nodules
OUTCOME	genotoxic lesions may cause stably inherited, aberrant DNA methylation patterns which may be responsible for site-specific hypomethylation of the c-myc protooncogene in liver nodules.
DISEASE	no information given
PUBMED	1859451
METHOD	Restriction digestion followed by southern blot

Record No:19

DB_ACCESS_NO	80018
REGION	entire gene
SEQUENCE	N/A
STATUS	pattern of methylation of the genes studied in the hybrid cells was a chimera of the parental patterns
CELL_LINE	somatic hybrid cell formed from HL-60 and B-lymphoid cells
OUTCOME	specific DNA methylation patterns from both parental cells are inherited in a chimeric pattern by the hybrid and are independent of gene expression.
DISEASE	myeloid maturation
PUBMED	1649355
METHOD	Restriction digestion followed by southern blot

Record No:20

DB_ACCESS_NO	80019
REGION	exon 3
SEQUENCE	CCGG
STATUS	hypomethylation as compared to controls
CELL_LINE	human myeloma cell lines compared with normal lymphocytes, Raji cells and LCL
OUTCOME	hypomethylation in the third exon of c-MYCmight be related to the enhanced expression of c-MYC in these human myeloma cell lines
DISEASE	myeloma
PUBMED	2004018
METHOD	Restriction digestion followed by southern blot

Record No:21

DB_ACCESS_NO	80020
REGION	entire gene
SEQUENCE	N/A
STATUS	COLO320DM: half are truncated and methylated, COLO320HSR normal and unmethylated
CELL_LINE	COLO320DM and COLO320HSR human cell lines compared with fibroblasts and granulocytes
OUTCOME	correlation between expression and the state of methylation for human c-myc oncogenes
DISEASE	no information given
PUBMED	2649153
METHOD	no information available

Record No:22

DB_ACCESS_NO	80021
REGION	entire gene specially exon and intron 2 and 3' region
SEQUENCE	N/A
STATUS	rapid changes in exon and intron 2 in both humans and mice overall there is similarity
CELL_LINE	Human liver samples and C57BL/6NJcl mice liver samples of different ages
OUTCOME	age-associated alteration of c-myc gene methylation is closely related to biological aging rather than to chronological aging
DISEASE	aging
PUBMED	2079963
METHOD	Restriction digestion followed by southern blot

Record No:23

DB_ACCESS_NO	80022
REGION	partially methylated regions flanking unmethylated domain -2 kb upstream of the 5' exon 1 to the 3'
SEQUENCE	N/A
STATUS	spleen: age-related hypomethylation both the 3' and 5' sides of the domain, liver: hypermethylation only at the 3' side
CELL_LINE	spleen and liver tissue from C57BL/ 6NJcl mice at different ages
OUTCOME	correlation between the changes of mRNA and DNA methylation
DISEASE	aging
PUBMED	2911270
METHOD	Restriction digestion followed by southern blot

Record No:24

DB_ACCESS_NO	80023
REGION	entire gene
SEQUENCE	CCGG
STATUS	c-myc locus variably methylated in all tissues, except in where erythrocytic DNA which showed no evidence of significant methylation of c-myc
CELL_LINE	tissues from adult Leghorn chickens
OUTCOME	c-myc is active in the later stages of henmtopoiesis.
DISEASE	no information given
PUBMED	2446622
METHOD	Restriction digestion followed by southern blot

Record No:25

DB_ACCESS_NO	80024
REGION	entire gene
SEQUENCE	N/A
STATUS	hypomethylation inn disease vs control and amplified in CLL
CELL_LINE	peripheral blood mononuclear cells (PBMC) from patients and control
OUTCOME	methylation at the c-myc gene changes during the crisis in CLL patients and could correlate with the process
DISEASE	human lymphoproliferative diseases
PUBMED	3480763
METHOD	Restriction digestion followed by southern blot

Record No:26

DB_ACCESS_NO	80025
REGION	entire gene specially intron 2 and exon 2
SEQUENCE	CCGG
STATUS	spleen: 3' end of intron 2 is hypomethylated, liver: exon 2 hypermethylated
CELL_LINE	spleen, liver and brain tissue from C57BL/6NJcl mice
OUTCOME	methylation changes are change is tissue- and DNA-region-specific during the process of aging
DISEASE	aging
PUBMED	3021154
METHOD	Restriction digestion followed by southern blot

Record No:27

DB_ACCESS_NO	80026
REGION	entire gene
SEQUENCE	CCGG
STATUS	no change in methylation status before or after treatment
CELL_LINE	human leukemic K-562 cell line before and after treatment
OUTCOME	c-myc mRNA expression remains unaffected
DISEASE	leukemia
PUBMED	2425967
METHOD	Restriction digestion followed by southern blot

Record No:28

DB_ACCESS_NO	80027
REGION	entire gene
SEQUENCE	Na
STATUS	paper not accessible
CELL_LINE	human promyelocytic leukemia cell line HL-60 before and after treatment
OUTCOME	paper not accessible
DISEASE	leukemia
PUBMED	3860729
METHOD	paper not accessible

Record No:29

DB_ACCESS_NO	80028
REGION	entire gene specially intron 2 and exon 2
SEQUENCE	CCGG
STATUS	hypomethylation in rearranged plasmacytoma as compared to control, hypermethylation in non-rearranged plasmacytoma
CELL_LINE	normal kidney and liver tissue vs plasmacytoma of mice
OUTCOME	housekeeping genes undermethylated in promoter regions
DISEASE	plasmacytoma
PUBMED	4092935
METHOD	Restriction digestion followed by southern blot

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For queries please contact Dr. Sudipto Saha (ssaha4@jcbose.ac.in, ssaha4@gmail.com)